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PPIH Polyclonal antibody

PPIH Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11651-1-AP

Synonyms

CYP 20, CYP20, CYPH, PPIase H, PPIH, Rotamase H, SnuCyp 20, USA CYP

Validation Data Gallery

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  • Western Blot (WB) analysis of U2OS cells using PPIH Polyclonal antibody (11651-1-AP)

    WB analysis of U2OS using 11651-1-AP

    U2OS cells were subjected to SDS PAGE followed by western blot with 11651-1-AP (PPIH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PPIH Polyclonal antibody (11651-1-AP)

    WB analysis using 11651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11651-1-AP (PPIH antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPIH Polyclonal antibody (11651-1-AP)

    IHC staining of human colon cancer using 11651-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11651-1-AP (PPIH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PPIH Polyclonal antibody (11651-1-AP)

    IHC staining of human colon cancer using 11651-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11651-1-AP (PPIH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PPIH Polyclonal antibody (11651-1-AP)

    IF Staining of PC-3 using 11651-1-AP

    Immunofluorescent analysis of PC-3 cells using 11651-1-AP (PPIH antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inU2OS cells, K-562 cells, PC-3 cells, mouse colon tissue, rat colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

11651-1-AP targets PPIH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIH fusion protein Ag2253
Full Name peptidylprolyl isomerase H (cyclophilin H)
Calculated Molecular Weight 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC003412
Gene Symbol PPIH
Gene ID (NCBI) 10465
RRIDAB_2284306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPIH(Peptidyl-prolyl cis-trans isomerase H) is also named as CYP20, CYPH and belongs to cyclophilin-type PPIase family, PPIase H subfamily.It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and may act as a chaperone(PMID:12875835).

Protocols

Product Specific Protocols
WB protocol for PPIH antibody 11651-1-APDownload protocol
IHC protocol for PPIH antibody 11651-1-APDownload protocol
IF protocol for PPIH antibody 11651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Theranostics

Dysregulation of interaction between LOXhigh fibroblast and smooth muscle cells contributes to the pathogenesis of aortic dissection.

Authors - Yinan Chen
View Article