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PPIL2 Polyclonal antibody

PPIL2 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 12590-2-AP

Synonyms

CYC4, Cyclophilin 60, Cyp 60, CYP60, hCyP 60, PPIase, PPIL2, Rotamase PPIL2

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using PPIL2 Polyclonal antibody (12590-2-AP)

    WB analysis of human kidney using 12590-2-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using PPIL2 Polyclonal antibody (12590-2-AP)

    WB analysis of mouse kidney using 12590-2-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PPIL2 Polyclonal antibody (12590-2-AP)

    WB analysis of HEK-293 using 12590-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12590-2-AP (PPIL2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PPIL2 Polyclonal antibody (12590-2-AP)

    IP experiment of HEK-293 using 12590-2-AP

    IP result of anti-PPIL2 (IP:12590-2-AP, 4ug; Detection:12590-2-AP 1:300) with HEK-293 cells lysate 2600 ug.

Tested Applications

Positive WB detected inhuman kidney tissue, HEK-293 cells, mouse kidney tissue
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12590-2-AP targets PPIL2 in WB, IP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL2 fusion protein Ag3296
Full Name peptidylprolyl isomerase (cyclophilin)-like 2
Calculated Molecular Weight 520 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC028385
Gene Symbol PPIL2
Gene ID (NCBI) 23759
RRIDAB_2169606
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPIL2 antibody 12590-2-APDownload protocol
IP protocol for PPIL2 antibody 12590-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Front Cardiovasc Med

Pilose Antler Peptide-3.2KD Ameliorates Adriamycin-Induced Myocardial Injury Through TGF-β/SMAD Signaling Pathway.

Authors - Yan Xu
View Article