PPIL4 Polyclonal antibody

PPIL4 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 12538-1-AP

Synonyms

Cyclophilin like protein PPIL4, HDCME13P, PPIase, PPIL4, Rotamase PPIL4



Tested Applications

Positive WB detected inHEK-293 cells, HCT 116 cells, mouse kidney tissue, HeLa cells, HepG2 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

12538-1-AP targets PPIL4 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPIL4 fusion protein Ag3249
Full Name peptidylprolyl isomerase (cyclophilin)-like 4
Calculated Molecular Weight 492 aa, 57 kDa
Observed Molecular Weight 58-65 kDa
GenBank Accession NumberBC016984
Gene Symbol PPIL4
Gene ID (NCBI) 85313
RRIDAB_10644441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPIL4 antibody 12538-1-APDownload protocol
IF protocol for PPIL4 antibody 12538-1-APDownload protocol
IP protocol for PPIL4 antibody 12538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

Up-regulation of RNA Binding Proteins Contributes to Folate Deficiency-Induced Neural Crest Cells Dysfunction.

Authors - Wenbo Liu