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  • KD/KO Validated

PPP1CB Polyclonal antibody

PPP1CB Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 55136-1-AP

Synonyms

PP1 Beta, PP 1B, PPP1CB, PPP1CD



Tested Applications

Positive WB detected inA549 cells, BxPC-3 cells, NIH/3T3 cells, SH-SY5Y cells, mouse brain tissue
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55136-1-AP targets PPP1CB in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name protein phosphatase 1, catalytic subunit, beta isoform
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37-40 kDa, 33 kDa
GenBank Accession NumberNM_002709
Gene Symbol PPP1CB
Gene ID (NCBI) 5500
RRIDAB_10837232
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

Protocols

Product Specific Protocols
WB protocol for PPP1CB antibody 55136-1-APDownload protocol
IF protocol for PPP1CB antibody 55136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Pathog

Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.

Authors - Zhanying Hu
  • KD Validated
humanWB

Exp Eye Res

PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.

Authors - Yang Guo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Balawant (Verified Customer) (08-23-2022)

I have used this antibody for western blot in mice tissue sample (colon tissue). it is working great.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: Colon tissue sample and caco2 cells