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  • KD/KO Validated

PPP2R5A Polyclonal antibody

PPP2R5A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12675-2-AP

Synonyms

B56A, PP2A B subunit isoform B alpha, PPP2R5A, PR61A, PR61alpha



Tested Applications

Positive WB detected inhuman ileum tissue, HeLa cells, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, mouse heart tissue, rat heart tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12675-2-AP targets PPP2R5A in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP2R5A fusion protein Ag3360
Full Name protein phosphatase 2, regulatory subunit B', alpha isoform
Calculated Molecular Weight 486 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC022474
Gene Symbol PPP2R5A
Gene ID (NCBI) 5525
RRIDAB_2252956
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for PPP2R5A antibody 12675-2-APDownload protocol
IHC protocol for PPP2R5A antibody 12675-2-APDownload protocol
IF protocol for PPP2R5A antibody 12675-2-APDownload protocol
IP protocol for PPP2R5A antibody 12675-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncol Rep

PMID: 28849187

Authors - Zehang Zhuang
  • KD Validated
humanWB

Oncol Lett

PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer.

Authors - Siwen Yin
humanWB

Cell Death Dis

Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration

Authors - Xiaoman Zhu