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  • KD/KO Validated

PPP3CB Polyclonal antibody

PPP3CB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13340-1-AP

Synonyms

CALNA2, CALNB, CAM PRP catalytic subunit, CNA2, PPP3CB



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, HEK-293 cells, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU-251 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13340-1-AP targets PPP3CB in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3CB fusion protein Ag4163
Full Name protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
Calculated Molecular Weight 525 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC028049
Gene Symbol PPP3CB
Gene ID (NCBI) 5532
RRIDAB_2284217
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PPP3CB antibody 13340-1-APDownload protocol
IHC protocol for PPP3CB antibody 13340-1-APDownload protocol
IF protocol for PPP3CB antibody 13340-1-APDownload protocol
IP protocol for PPP3CB antibody 13340-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Gastroenterology

Regulator of Calcineurin 1 Gene Isoform 4, Downregulated in Hepatocellular Carcinoma, Prevents Proliferation, Migration, and Invasive Activity of Cancer Cells and Growth of Orthotopic Tumors by Inhibiting Nuclear Translocation of NFAT1.

Authors - Haojie Jin
humanIP

Cell Death Differ

The Smad4-MYO18A-PP1A complex regulates β-catenin phosphorylation and pemigatinib resistance by inhibiting PAK1 in cholangiocarcinoma.

Authors - Jialiang Liu
humanWB

Oncogene

Regulator of calcineurin 1 gene isoform 4 in pancreatic ductal adenocarcinoma regulates the progression of tumor cells.

Authors - Mengyi Lao
  • KD Validated

Sci Rep

Calcineurin regulates cyclin D1 stability through dephosphorylation at T286.

Authors - Takahiro Goshima
humanWB

Proteomics

Analysis of EV71 infection progression using triple-SILAC-based proteomics approach.

Authors - Hao-Yu Li
mouseWB

Eur J Pharmacol

Stachydrine hydrochloride suppresses phenylephrine-induced pathological cardiac hypertrophy by inhibiting the calcineurin/nuclear factor of activated T-cell signalling pathway.

Authors - Jiali Zheng