PPP3R1 Polyclonal antibody

PPP3R1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13210-1-AP

Synonyms

Calcineurin subunit B type 1, CALNB1, CNA2, CNB, CNB1, PPP3R1



Tested Applications

Positive WB detected inK-562 cells, human stomach tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13210-1-AP targets PPP3R1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PPP3R1 fusion protein Ag3992
Full Name protein phosphatase 3 (formerly 2B), regulatory subunit B, alpha isoform
Calculated Molecular Weight 170 aa, 19 kDa
Observed Molecular Weight 19 kDa
GenBank Accession NumberBC027913
Gene Symbol PPP3R1
Gene ID (NCBI) 5534
RRIDAB_2252760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Protein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R has two isoforms, PPP3R1 and PPP3R2. PPP3Rl is ubiquitously expressed in different tissues and is coded by the PPP3R1 gene which was mapped recently to human chromosome 2. PPP3Rl has four functional calcium-binding sites.

Protocols

Product Specific Protocols
WB protocol for PPP3R1 antibody 13210-1-APDownload protocol
IHC protocol for PPP3R1 antibody 13210-1-APDownload protocol
IP protocol for PPP3R1 antibody 13210-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Acta Biomater

Substrate stiffness regulates differentiation of induced pluripotent stem cells into heart valve endothelial cells.

Authors - Hong Cao
mouseWB

Proc Natl Acad Sci U S A

Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries.

Authors - Xiju He
humanWB

Biochem Biophys Res Commun

The calcineurin B subunit induces TNF-related apoptosis-inducing ligand (TRAIL) expression via CD11b-NF-κB pathway in RAW264.7 macrophages.

Authors - Su Zhenyi Z
chickenWB

Biol Trace Elem Res

Transcriptional Study Revealed That Boron Supplementation May Alter the Immune-Related Genes Through MAPK Signaling in Ostrich Chick Thymus.

Authors - Ke Xiao
mouseWB

Autophagy

Narirutin activates TFEB (transcription factor EB) to protect against acetaminophen-induced liver injury by targeting PPP3/calcineurin

Authors - Zhiyuan Fang
humanIF

Autophagy

SMURF1 controls the PPP3/calcineurin complex and TFEB at a regulatory node for lysosomal biogenesis

Authors - Qin Xia