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PRDM7 Polyclonal antibody

PRDM7 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22078-1-AP

Synonyms

PFM4, PR domain containing 7, PR domain containing protein 7, PRDM7, PRDM9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PRDM7 Polyclonal antibody (22078-1-AP)

    WB analysis of HeLa using 22078-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22078-1-AP (PRDM7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRDM7 Polyclonal antibody (22078-1-AP)

    IHC staining of mouse testis using 22078-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 22078-1-AP (PRDM7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22078-1-AP is PRDM7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDM7 fusion protein Ag17226
Full Name PR domain containing 7
Calculated Molecular Weight 171 aa, 18, 46 kDa
Observed Molecular Weight 46 kDa, 66 kDa
GenBank Accession NumberBC107033
Gene Symbol PRDM7
Gene ID (NCBI) 11105
RRIDAB_2878986
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRDM7 antibody 22078-1-APDownload protocol
IHC protocol for PRDM7 antibody 22078-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols