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  • KD/KO Validated

PRDX1 Polyclonal antibody

PRDX1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 15816-1-AP

Synonyms

MSP23, NKEF A, NKEFA, PAG, PAGA, PAGB, peroxiredoxin 1, PRDX1, PRX1, PRXI, TDPX2, Thioredoxin peroxidase 2



Tested Applications

Positive WB detected inA549 cells, HEK-293 cells, U2OS cells, HeLa cells, HepG2 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman breast cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, RAW 264.7 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15816-1-AP targets PRDX1 in WB, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRDX1 fusion protein Ag8602
Full Name peroxiredoxin 1
Calculated Molecular Weight 199 aa, 22 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC007063
Gene Symbol PRDX1
Gene ID (NCBI) 5052
RRIDAB_2170318
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX1(Peroxiredoxin-1), also named as PAGA, PAGB, TDPX2, PAG or NKEF-A, belongs to the ahpC/TSA family. PRDX1 is a thiol reductase that plays critical roles in oxidative and thermal stress defense mechanisms through its abilities to metabolize H2O2 and act as a molecular chaperone, respectively. PRDX1 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2 (PMID: 9497357). It reduces an intramolecular disulfide bond in GDPD5 that gates the ability to GDPD5 to drive postmitotic motor neuron differentiation. PRDX1 can form a dimer, and also can be phosphorylated on Thr-90 during the M-phase, which leads to a more than 80% decrease in enzymatic activity (PMID: 22583657, 11986303).

Protocols

Product Specific Protocols
WB protocol for PRDX1 antibody 15816-1-APDownload protocol
IHC protocol for PRDX1 antibody 15816-1-APDownload protocol
IF protocol for PRDX1 antibody 15816-1-APDownload protocol
FC protocol for PRDX1 antibody 15816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Suppression of mitochondrial ROS by prohibitin drives glioblastoma progression and therapeutic resistance.

Authors - Haohao Huang
mouseWB

Redox Biol

PMID: 32604037

Authors - Anindya Roy Chowdhury
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei

Cell Chem Biol

Parvifoline AA Promotes Susceptibility of Hepatocarcinoma to Natural Killer Cell-Mediated Cytolysis by Targeting Peroxiredoxin.

Authors - Huifang Zhu
mouseWB

Cell Rep

The Ets transcription factor GABP is a component of the hippo pathway essential for growth and antioxidant defense.

Authors - Wu Hongtan H
humanIHC

Front Immunol

A novel cuproptosis pattern and tumor immune microenvironment characterization in urothelial carcinoma of the bladder

Authors - Huan Feng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guillermo (Verified Customer) (07-20-2022)

Very nice product.

  • Applications: Western Blot
FH

Wei (Verified Customer) (03-12-2020)

PRDX1 is easily detected in mouse hearts. It has strong, and clear band.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart