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  • Featured Product
  • KD/KO Validated

peroxiredoxin 2 Polyclonal antibody

peroxiredoxin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10545-2-AP

Synonyms

NKEF B, NKEFB, peroxiredoxin 2, PRDX2, PRP, PRX II, PRX2, PRXII, TDPX1, Thioredoxin peroxidase 1, TSA

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    WB analysis using 10545-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    WB analysis of HepG2 using 10545-2-AP

    WB result of peroxiredoxin 2 antibody (10545-2-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-peroxiredoxin 2 transfected HepG2 cells.

  • Immunoprecipitation (IP) experiment of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IP experiment of HepG2 using 10545-2-AP

    IP result of anti-peroxiredoxin 2 (IP:10545-2-AP, 4ug; Detection:10545-2-AP 1:4000) with HepG2 cells lysate 960 ug.

  • Immunohistochemistry (IHC) staining of human liver tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human liver using 10545-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human liver using 10545-2-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human hepatocirrhosis using 10545-2-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IHC staining of human hepatocirrhosis using 10545-2-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10545-2-AP (peroxiredoxin 2 antibody) at dilution of 1:600 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IF Staining of HepG2 using 10545-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using peroxiredoxin 2 Polyclonal antibody (10545-2-AP)

    IF Staining of HeLa using 10545-2-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using peroxiredoxin 2 antibody (10545-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, C2C12 cells, PC-12 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver tissue, human hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:600-1:3000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10545-2-AP targets peroxiredoxin 2 in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen peroxiredoxin 2 fusion protein Ag0835
Full Name peroxiredoxin 2
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC003022
Gene Symbol PRDX2
Gene ID (NCBI) 7001
RRIDAB_2168202
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRDX2, also named as TSA, PRP, NKEFB and TDPX1, belongs to the ahpC/TSA family. It is known to act as an antioxidant enzyme whose main function is H2O2 reduction in cells. PRDX2 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system. It may play an important role in eliminating peroxides generated during metabolism. PRDX2 might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. PRDX2 actions may be related to the expression of NFKB and IKB.(PMID:21248284)

Protocols

Product Specific Protocols
WB protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IHC protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IF protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
IP protocol for peroxiredoxin 2 antibody 10545-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

ACS Nano

Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.

Authors - Zhiwei Liao
View Article
ratWB

Br J Pharmacol

Luteolin ameliorates rat myocardial ischemia-reperfusion injury through peroxiredoxin II activation.

Authors - Bo Wei
View Article
humanWB, IHC

Oncogene

PMID: 26500057

Authors - Y-H Park
View Article
human,mouseWB,IP,CoIP

Free Radic Biol Med

Oxidation resistance 1 regulates post-translational modifications of peroxiredoxin 2 in the cerebellum.

Authors - Daria M Svistunova
View Article
mouseIF

Stem Cells

Dominant role of peroxiredoxin/JNK axis in stemness regulation during neurogenesis from embryonic stem cells.

Authors - Sun-Uk Kim
View Article
humanWB,IHC

Oxid Med Cell Longev

Azelaic Acid Exerts Antileukemia Effects against Acute Myeloid Leukemia by Regulating the Prdxs/ROS Signaling Pathway.

Authors - Dongdong Zhang
View Article