- Featured Product
- KD/KO Validated
PREB Polyclonal antibody
PREB Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 10146-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | SKOV-3 cells, 3T3-L1 cells , HT-1080 cells, HepG2 cells |
| Positive IP detected in | SKOV-3 cells |
| Positive IHC detected in | human prostate cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 6 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
10146-2-AP targets PREB in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PREB fusion protein Ag0202 |
| Full Name | prolactin regulatory element binding |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC002765 |
| Gene Symbol | PREB |
| Gene ID (NCBI) | 10113 |
| RRID | AB_2168779 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PREB antibody 10146-2-AP | Download protocol |
| IHC protocol for PREB antibody 10146-2-AP | Download protocol |
| IF protocol for PREB antibody 10146-2-AP | Download protocol |
| IP protocol for PREB antibody 10146-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis | ||
Int J Biol Macromol Cellular OCIAD2 protein is a proviral factor for hepatitis C virus replication. | ||
J Cell Sci A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.
| ||
Biochem Biophys Res Commun Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation. | ||
Biochim Biophys Acta Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis. | ||
Virol J PREB inhibits the replication of prototype foamy virus by affecting its transcription
|
