Perforin Polyclonal antibody

Perforin Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 14580-1-AP

Synonyms

Cytolysin, FLH2, HPLH2, P1, Perforin, Perforin 1, PFN1, PFP, PRF1



Tested Applications

Positive WB detected inmouse kidney tissue, mouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Sample-dependent, check data in validation data gallery

Product Information

14580-1-AP targets Perforin in WB, IF,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity chicken, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Perforin fusion protein Ag6060
Full Name perforin 1 (pore forming protein)
Calculated molecular weight 61 kDa
Observed molecular weight 70-75 kDa
GenBank accession numberBC063043
Gene symbol PRF1
Gene ID (NCBI) 5551
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Perforin 1 (PRF1) is one of the major cytolytic proteins of cytolytic granules. It is known to be a crucial effector molecule in cytolytic T lymphocyte and natural killer cell-mediated cytotoxicity. This protein has structural and functional similarities to complement component C9. Like C9, this protein creates transmembrane tubules and is capable of lysing nonspecifically a variety of target cells. Defects in PRF1 are the cause of familial hemophagocytic lymphohistiocytosis type 2 (FHL2), which is characterized by immune dysregulation with hypercytokinemia and defective natural killer cell function. (PMID: 2417226; 7774276; 2783486; 10583959)

Protocols

Product Specific Protocols
WB protocol for Perforin antibody 14580-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
chickenWB

Res Vet Sci

Characterization of cytotoxicity-related gene expression in response to virulent Marek's disease virus infection in the bursa of Fabricius.

Authors - Cui-Ying Chen
humanWB

Mol Immunol

VEGF165b augments NK92 cytolytic activity against human K562 leukemia cells by upregulating the levels of perforin and granzyme B via the VEGR1-PLC pathway.

Authors - Xiaoyin Wang
humanIF, WB

Front Cell Infect Microbiol

The Role of Tim-3 on dNK Cells Dysfunction During Abnormal Pregnancy With Toxoplasma gondii Infection.

Authors - Teng Li