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PRH1 Polyclonal antibody

PRH1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 21460-1-AP

Synonyms

Db F, Db s, Pa, Parotid acidic protein, Parotid double band protein, PIF F, PIF S, Pr1/Pr2, PRH1, PRH1/2, PRH2, Protein A Peptide P C, Protein C, PRP 1/PRP 2, PRP 3/PRP 4

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PRH1 Polyclonal antibody (21460-1-AP)

    WB analysis of HepG2 using 21460-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21460-1-AP (PRH1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRH1 Polyclonal antibody (21460-1-AP)

    IF Staining of HepG2 using 21460-1-AP

    Immunofluorescent analysis of HepG2 cells using 21460-1-AP (PRH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21460-1-AP is PRH1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRH1 fusion protein Ag14833
Full Name proline-rich protein HaeIII subfamily 1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC064553
Gene Symbol PRH1
Gene ID (NCBI) 5554
RRIDAB_2878862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRH1, also known as Salivary acidic proline-rich phosphoprotein 1/2, is a 166 amino acid secreted protein whose molecular weight is 17 kDa. It functions as a highly potent inhibitor of calcium phosphate crystal growth. Similar to other PRPs, PRH1 provides a protective and reparative environment for dental enamel, which is important for teeth integrity. This antibody specifically recognises the 17 kDa protein.

Protocols

Product Specific Protocols
WB protocol for PRH1 antibody 21460-1-APDownload protocol
IF protocol for PRH1 antibody 21460-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols