• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PRLR Monoclonal antibody

PRLR Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E10G5

Cat no : 67292-1-Ig

Synonyms

hPRLrI, PRL R, PRLR, prolactin receptor

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PRLR Monoclonal antibody (67292-1-Ig)

    WB analysis using 67292-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using PRLR Monoclonal antibody (67292-1-Ig)

    WB analysis of A431 using 67292-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of T-47D cells using PRLR Monoclonal antibody (67292-1-Ig)

    WB analysis of T-47D using 67292-1-Ig

    T-47D cells were subjected to SDS PAGE followed by western blot with 67292-1-Ig (PRLR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRLR Monoclonal antibody (67292-1-Ig)

    IHC staining of human breast cancer using 67292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRLR Monoclonal antibody (67292-1-Ig)

    IHC staining of human breast cancer using 67292-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67292-1-Ig (PRLR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inJurkat cells, A431 cells, T-47D cells, 4T1 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67292-1-Ig targets PRLR in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRLR fusion protein Ag28778
Full Name prolactin receptor
Calculated Molecular Weight 622 aa, 70 kDa
Observed Molecular Weight 45 kDa, 70 kDa, 85-85 kDa
GenBank Accession NumberBC059392
Gene Symbol PRLR
Gene ID (NCBI) 5618
RRIDAB_2882557
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PRLR belongs to the cytokine type I subfamily, which also includes receptors for leptin, leukemia inhibiting factor, erythropoietin and so on. PRLR has long and short forms at approximately 70 and 45 kDa, respectively, and the mature, glycosylated form of PRLR migrates at 85-95 kDa in human decidua and placenta (PMID: 18258683).

Protocols

Product Specific Protocols
WB protocol for PRLR antibody 67292-1-IgDownload protocol
IHC protocol for PRLR antibody 67292-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Genet

What Mediates Fibrosis in the Tumor Microenvironment of Clear Renal Cell Carcinoma.

Authors - Wenbo Yang
View Article
WB

Front Vet Sci

Hybrid Broussonetia papyrifera Fermented Feed Can Play a Role Through Flavonoid Extracts to Increase Milk Production and Milk Fatty Acid Synthesis in Dairy Goats.

Authors - Mengjie Zhao
View Article