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PRPF40A Polyclonal antibody

PRPF40A Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 17392-1-AP

Synonyms

Fas ligand associated factor 1, FBP 11, FBP11, FLAF1, FNBP3, Formin binding protein 11, Formin binding protein 3, HIP 10, HIP10, Huntingtin yeast partner A, HYPA, NY REN 6, PRPF40A

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of HEK-293 using 17392-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of HeLa using 17392-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of HeLa using 17392-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PRPF40A Polyclonal antibody (17392-1-AP)

    WB analysis of mouse brain using 17392-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17392-1-AP (PRPF40A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PRPF40A Polyclonal antibody (17392-1-AP)

    IP experiment of HEK-293 using 17392-1-AP

    IP result of anti-PRPF40A (IP:17392-1-AP, 4ug; Detection:17392-1-AP 1:500) with HEK-293 cells lysate 3000ug.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17392-1-AP targets PRPF40A in WB, IP,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF40A fusion protein Ag11290
Full Name PRP40 pre-mRNA processing factor 40 homolog A (S. cerevisiae)
Calculated Molecular Weight 957 aa, 109 kDa
Observed Molecular Weight 130-140 kDa
GenBank Accession NumberBC027178
Gene Symbol PRPF40A
Gene ID (NCBI) 55660
RRIDAB_2171031
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRPF40A antibody 17392-1-APDownload protocol
IP protocol for PRPF40A antibody 17392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols