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PRTN3 Monoclonal antibody

PRTN3 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2D1B8

Cat no : 67030-1-Ig

Synonyms

ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PRTN3 Monoclonal antibody (67030-1-Ig)

    WB analysis of human placenta using 67030-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human spleen tissue using PRTN3 Monoclonal antibody (67030-1-Ig)

    IHC staining of human spleen using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PRTN3 Monoclonal antibody (67030-1-Ig)

    IHC staining of human spleen using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Monoclonal antibody (67030-1-Ig)

    IHC staining of human tonsillitis using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Monoclonal antibody (67030-1-Ig)

    IHC staining of human tonsillitis using 67030-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67030-1-Ig (PRTN3 antibody) at dilution of 1:2000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using PRTN3 Monoclonal antibody (67030-1-Ig)

    IF Staining of human tonsillitis using 67030-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PRTN3 antibody (67030-1-Ig, Clone: 2D1B8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67030-1-Ig targets PRTN3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PRTN3 fusion protein Ag25915
Full Name proteinase 3
Calculated Molecular Weight 256 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC096183
Gene Symbol PRTN3
Gene ID (NCBI) 5657
RRIDAB_2882345
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PRTN3 antibody 67030-1-IgDownload protocol
IHC protocol for PRTN3 antibody 67030-1-IgDownload protocol
IF protocol for PRTN3 antibody 67030-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols