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PSMB10 Polyclonal antibody

PSMB10 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15976-1-AP

Synonyms

beta2i, LMP10, Low molecular mass protein 10, Macropain subunit MECl 1, MECL1, Proteasome MECl 1, Proteasome subunit beta 2i, PSMB10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB10 Polyclonal antibody (15976-1-AP)

    WB analysis using 15976-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15976-1-AP (PSMB10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using PSMB10 Polyclonal antibody (15976-1-AP)

    WB analysis of human liver using 15976-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 15976-1-AP (PSMB10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB10 Polyclonal antibody (15976-1-AP)

    IHC staining of human liver cancer using 15976-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15976-1-AP (PSMB10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB10 Polyclonal antibody (15976-1-AP)

    IHC staining of human liver cancer using 15976-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15976-1-AP (PSMB10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRaji cells, human liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15976-1-AP targets PSMB10 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMB10 fusion protein Ag8764
Full Name proteasome (prosome, macropain) subunit, beta type, 10
Calculated Molecular Weight 273 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC017198
Gene Symbol PSMB10
Gene ID (NCBI) 5699
RRIDAB_2171872
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proteasome subunit, beta type 10 (PSMB10) gene regulated by interferon-gamma is a core part of the 26S proteasome complex, which is an important protein degrading system. Study identifies immunosubunit PSMB10 as a novel regulator that contributes to Ang II-induced atrial fibrillation (AF) and suggests that inhibition of PSMB10 may represent a potential therapeutic target for treating hypertensive AF. (PMID: 29507100, PMID: 16965406)

Protocols

Product Specific Protocols
WB protocol for PSMB10 antibody 15976-1-APDownload protocol
IHC protocol for PSMB10 antibody 15976-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Pharmacol

Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.

Authors - Xin Xie
View Article
WB

Metab Brain Dis

Molecular alteration of the proteasome contributes to AD-like pathology in the brain of HFD-STZ diabetic rats

Authors - Han Gao
View Article
WB

Biochem Pharmacol

Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP)

Authors - Xin Xie
View Article