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PSMB10 Monoclonal antibody

PSMB10 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, pig, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1G8C1

Cat no : 66403-1-Ig

Synonyms

beta2i, LMP10, Low molecular mass protein 10, Macropain subunit MECl 1, MECL1, Proteasome MECl 1, Proteasome subunit beta 2i, PSMB10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis using 66403-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of Raji cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of Raji using 66403-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat thymus tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of rat thymus using 66403-1-Ig

    rat thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Ramos cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of Ramos using 66403-1-Ig

    Ramos cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of U-937 using 66403-1-Ig

    U-937 cells were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human spleen tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of human spleen using 66403-1-Ig

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig thymus tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    WB analysis of pig thymus using 66403-1-Ig

    pig thymus tissue were subjected to SDS PAGE followed by western blot with 66403-1-Ig (PSMB10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    IHC staining of human liver cancer using 66403-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB10 Monoclonal antibody (66403-1-Ig)

    IHC staining of human liver cancer using 66403-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66403-1-Ig (PSMB10 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRaji cells, Ramos cells, U-937 cells, rat thymus tissue, human spleen tissue, pig thymus tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66403-1-Ig targets PSMB10 in WB, IHC, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB10 fusion protein Ag8835
Full Name proteasome (prosome, macropain) subunit, beta type, 10
Calculated Molecular Weight 273 aa, 29 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC017198
Gene Symbol PSMB10
Gene ID (NCBI) 5699
RRIDAB_2881777
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMB10(Proteasome subunit beta type-10) is also named as LMP10, MECL1.It belongs to the peptidase T1B family and is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.

Protocols

Product Specific Protocols
WB protocol for PSMB10 antibody 66403-1-IgDownload protocol
IHC protocol for PSMB10 antibody 66403-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols