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PSMB9 Monoclonal antibody

PSMB9 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C7C8

Cat no : 67748-1-Ig

Synonyms

beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB9 Monoclonal antibody (67748-1-Ig)

    WB analysis using 67748-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Western Blot (WB) analysis of rat spleen tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    WB analysis of rat spleen using 67748-1-Ig

    rat spleen tissue were subjected to SDS PAGE followed by western blot with 67748-1-Ig (PSMB9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human colon cancer using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human colon cancer using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PSMB9 Monoclonal antibody (67748-1-Ig)

    IHC staining of human spleen using 67748-1-Ig

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67748-1-Ig (PSMB9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inRaji cells, rat spleen tissue, Ramos cells, Daudi cells, HL-60 cells, THP-1 cells
Positive IHC detected inhuman colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67748-1-Ig targets PSMB9 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PSMB9 fusion protein Ag6802
Full Name proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
Calculated Molecular Weight 23 kDa
Observed Molecular Weight20-23 kDa
GenBank Accession NumberBC065513
Gene Symbol PSMB9
Gene ID (NCBI) 5698
RRIDAB_2918517
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PSMB9 antibody 67748-1-IgDownload protocol
IHC protocol for PSMB9 antibody 67748-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols