PSMC4 Polyclonal antibody
PSMC4 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 11389-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
11389-1-AP targets PSMC4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSMC4 fusion protein Ag1943 |
| Full Name | proteasome (prosome, macropain) 26S subunit, ATPase, 4 |
| Calculated Molecular Weight | 418 aa, 47 kDa |
| Observed Molecular Weight | 43-50 kDa |
| GenBank Accession Number | BC014488 |
| Gene Symbol | PSMC4 |
| Gene ID (NCBI) | 5704 |
| RRID | AB_2300373 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMC4(proteasome (prosome, macropain) 26S subunit, ATPase, 4) is also named as TBP7, S6, TBP7, MIP224 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The predicted 458-amino acid TBP7 protein is 57% identical to TBP1 and they can form heterodimers(PMID:8419915).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMC4 antibody 11389-1-AP | Download protocol |
| IHC protocol for PSMC4 antibody 11389-1-AP | Download protocol |
| IF protocol for PSMC4 antibody 11389-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Neuron Antibody Therapy Targeting RAN Proteins Rescues C9 ALS/FTD Phenotypes in C9orf72 Mouse Model. | ||
Nutrients Role of Epigallocatechin Gallate in Glucose, Lipid, and Protein Metabolism and L-Theanine in the Metabolism-Regulatory Effects of Epigallocatechin Gallate. | ||
Cells The Prostaglandin E2 Receptor EP4 Promotes Vascular Neointimal Hyperplasia through Translational Control of Tenascin C via the cAPM/PKA/mTORC1/rpS6 Pathway |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Tatyana (Verified Customer) (03-11-2023) | Was tested in ICC on PFA-fixed human cells. Provides a signal that looks specific (as compared to no primary control). 4% PFA followed by methanol permebealisation; antibody prepared in 5% goat serum in PBST, overnight incubation (no blocking step).
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