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PTPLAD1 Polyclonal antibody

PTPLAD1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28572-1-AP

Synonyms

B IND1, BIND1, Butyrate induced protein 1, FLJ90376, HACD3, hB ind1, HSPC121, PTPLAD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTPLAD1 Polyclonal antibody (28572-1-AP)

    WB analysis using 28572-1-AP

    Human placenta tissue were subjected to SDS PAGE followed by western blot with 28572-1-AP (PTPLAD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPLAD1 Polyclonal antibody (28572-1-AP)

    IHC staining of human breast cancer using 28572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28572-1-AP (PTPLAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PTPLAD1 Polyclonal antibody (28572-1-AP)

    IHC staining of human breast cancer using 28572-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28572-1-AP (PTPLAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PTPLAD1 Polyclonal antibody (28572-1-AP)

    IHC staining of human liver cancer using 28572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28572-1-AP (PTPLAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PTPLAD1 Polyclonal antibody (28572-1-AP)

    IHC staining of human liver cancer using 28572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28572-1-AP (PTPLAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PTPLAD1 Polyclonal antibody (28572-1-AP)

    IHC staining of human liver cancer using 28572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28572-1-AP (PTPLAD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PTPLAD1 Polyclonal antibody (28572-1-AP)

    IHC staining of human liver cancer using 28572-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28572-1-AP (PTPLAD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28572-1-AP targets PTPLAD1 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPLAD1 fusion protein Ag29557
Full Name protein tyrosine phosphatase-like A domain containing 1
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberNM_016395
Gene Symbol PTPLAD1
Gene ID (NCBI) 51495
RRIDAB_2881172
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTPLAD1 antibody 28572-1-APDownload protocol
IHC protocol for PTPLAD1 antibody 28572-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols