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  • Featured Product
  • KD/KO Validated

PTPN14 Polyclonal antibody

PTPN14 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18053-1-AP

Synonyms

PEZ, PTP36, PTPN14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PTPN14 Polyclonal antibody (18053-1-AP)

    WB analysis using 18053-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PTPN14 Polyclonal antibody (18053-1-AP)

    WB analysis of HeLa using 18053-1-AP

    WB result of PTPN14 antibody (18053-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPN14 transfected HeLa cells.

  • Western Blot (WB) analysis of HeLa cells using PTPN14 Polyclonal antibody (18053-1-AP)

    WB analysis of HeLa using 18053-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using PTPN14 Polyclonal antibody (18053-1-AP)

    WB analysis of K-562 using 18053-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18053-1-AP (PTPN14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human placenta using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human placenta using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human skin using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human skin using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human spleen using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human spleen using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human lung using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human lung using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human ovary using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human ovary using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human brain using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human brain using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human kidney using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PTPN14 Polyclonal antibody (18053-1-AP)

    IHC staining of human kidney using 18053-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18053-1-AP (PTPN14 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

18053-1-AP targets PTPN14 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitycanine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTPN14 fusion protein Ag12571
Full Name protein tyrosine phosphatase, non-receptor type 14
Calculated Molecular Weight 1187 aa, 135 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC101754
Gene Symbol PTPN14
Gene ID (NCBI) 5784
RRIDAB_2284584
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN14 (tyrosine-protein phosphatase non-receptor type 14) is also named as PEZ and belongs to the protein-tyrosine phosphatase family and non-receptor class subfamily. It is a multi-domain protein expressed in breast, kidney, skeletal muscle, lung, placenta, and characterized by an N-terminal FERM domain and a C-terminal PTP domain with an intervening sequence containing an acidic region and a putative SH3 domain-binding sequence (PMID: 21701840). Defects in PTPN14 are a cause of choanal atresia and lymphedema (CHATLY).

Protocols

Product Specific Protocols
WB protocol for PTPN14 antibody 18053-1-APDownload protocol
IHC protocol for PTPN14 antibody 18053-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
canineWB

Biologicals

CDC20 is a potential target gene to inhibit the tumorigenesis of MDCK cells

Authors - Zhenbin Liu
View Article