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PTTG1IP Polyclonal antibody

PTTG1IP Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12575-1-AP

Synonyms

C21orf1, C21orf3, PBF, PTTG binding factor, PTTG1IP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PTTG1IP Polyclonal antibody (12575-1-AP)

    WB analysis using 12575-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12575-1-AP (PTTG1IP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PTTG1IP Polyclonal antibody (12575-1-AP)

    IP experiment of HEK-293 using 12575-1-AP

    IP result of anti-PTTG1IP (IP:12575-1-AP, 4ug; Detection:12575-1-AP 1:300) with HEK-293 cells lysate 2600 ug.

  • Immunohistochemistry (IHC) staining of human testis tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human testis using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human testis using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human colon using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human colon using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human liver using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PTTG1IP Polyclonal antibody (12575-1-AP)

    IHC staining of human liver using 12575-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 12575-1-AP (PTTG1IP antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTTG1IP Polyclonal antibody (12575-1-AP)

    IF Staining of HeLa using 12575-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTTG1IP antibody (12575-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PTTG1IP Polyclonal antibody (12575-1-AP)

    IF Staining of PC-3 using 12575-1-AP

    Immunofluorescent analysis of PC-3 cells using 12575-1-AP (PTTG1IP antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA375 cells, HEK-293 cells, HeLa cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman testis tissue, human colon tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12575-1-AP targets PTTG1IP in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PTTG1IP fusion protein Ag3250
Full Name pituitary tumor-transforming 1 interacting protein
Calculated Molecular Weight 180 aa, 20 kDa
Observed Molecular Weight 26 kDa
GenBank Accession NumberBC012858
Gene Symbol PTTG1IP
Gene ID (NCBI) 754
RRIDAB_10644145
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PTTG1IP antibody 12575-1-APDownload protocol
IHC protocol for PTTG1IP antibody 12575-1-APDownload protocol
IF protocol for PTTG1IP antibody 12575-1-APDownload protocol
IP protocol for PTTG1IP antibody 12575-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Horm Metab Res

MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line.

Authors - Wang Zhen
View Article
humanIHC

Oncol Lett

Expression of proteins associated with epithelial-mesenchymal transition in esophageal squamous cell carcinoma.

Authors - Tian Zhang
View Article
humanWB

Pharmacol Res

PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma

Authors - Wenfang Bao
View Article