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  • Featured Product
  • KD/KO Validated

PUS1 Polyclonal antibody

PUS1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11512-1-AP

Synonyms

MLASA, pseudouridylate synthase 1, PUS1, tRNA uridine isomerase I

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis of mouse lung using 11512-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis of HEK-293 using 11512-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PUS1 Polyclonal antibody (11512-1-AP)

    WB analysis of HeLa using 11512-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11512-1-AP (PUS1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PUS1 Polyclonal antibody (11512-1-AP)

    IHC staining of human ovary tumor using 11512-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PUS1 Polyclonal antibody (11512-1-AP)

    IHC staining of human ovary tumor using 11512-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11512-1-AP (PUS1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PUS1 Polyclonal antibody (11512-1-AP)

    IF Staining of MCF-7 using 11512-1-AP

    Immunofluorescent analysis of MCF-7 cells using 11512-1-AP (PUS1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse lung tissue, HEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11512-1-AP targets PUS1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PUS1 fusion protein Ag2071
Full Name pseudouridylate synthase 1
Calculated Molecular Weight 427 aa, 47 kDa
Observed Molecular Weight 47 kDa
GenBank Accession NumberBC019320
Gene Symbol PUS1
Gene ID (NCBI) 80324
RRIDAB_2284652
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PUS1 antibody 11512-1-APDownload protocol
IHC protocol for PUS1 antibody 11512-1-APDownload protocol
IF protocol for PUS1 antibody 11512-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

RNA

Mapping of pseudouridine residues on viral and cellular transcripts using a novel antibody-based technique.

Authors - Cecilia Martinez Campos
View Article
humanWB

Pharmgenomics Pers Med

PUS1 May Be a Potential Prognostic Biomarker and Therapeutic Target for Hepatocellular Carcinoma

Authors - Chenlu Lan
  • KD Validated
View Article