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PXMP2 Polyclonal antibody

PXMP2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24801-1-AP

Synonyms

Peroxisomal membrane protein 2, PMP22, PXMP2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of rat liver tissue using PXMP2 Polyclonal antibody (24801-1-AP)

    WB analysis of rat liver using 24801-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24801-1-AP (PXMP2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using PXMP2 Polyclonal antibody (24801-1-AP)

    WB analysis of rat liver using 24801-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 24801-1-AP (PXMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PXMP2 Polyclonal antibody (24801-1-AP)

    WB analysis of mouse liver using 24801-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 24801-1-AP (PXMP2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PXMP2 Polyclonal antibody (24801-1-AP)

    IHC staining of human liver using 24801-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24801-1-AP (PXMP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using PXMP2 Polyclonal antibody (24801-1-AP)

    IHC staining of human liver using 24801-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 24801-1-AP (PXMP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inrat liver tissue, mouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 24801-1-AP is PXMP2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PXMP2 fusion protein Ag20590
Full Name peroxisomal membrane protein 2, 22kDa
Calculated Molecular Weight 195 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC073997
Gene Symbol PXMP2
Gene ID (NCBI) 5827
RRIDAB_2879733
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PXMP2 antibody 24801-1-APDownload protocol
IHC protocol for PXMP2 antibody 24801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Biochim Biophys Acta Biomembr

Deciphering the potential involvement of PXMP2 and PEX11B in hydrogen peroxide permeation across the peroxisomal membrane reveals a role for PEX11B in protein sorting.

Authors - Celien Lismont
View Article
human,mouseWB

Nat Commun

Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA

Authors - Mirjana Malnar Črnigoj
View Article