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  • Featured Product
  • KD/KO Validated

PYCR1 Polyclonal antibody

PYCR1 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 22150-1-AP

Synonyms

P5C, P5C reductase 1, P5CR, P5CR 1, PIG45, PP222, PYCR, PYCR1

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using PYCR1 Polyclonal antibody (22150-1-AP)

    WB analysis of HeLa using 22150-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PYCR1 Polyclonal antibody (22150-1-AP)

    WB analysis of COLO 320 using 22150-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 22150-1-AP (PYCR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using PYCR1 Polyclonal antibody (22150-1-AP)

    IP experiment of HeLa using 22150-1-AP

    IP result of anti-PYCR1 (IP:22150-1-AP, 4ug; Detection:22150-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PYCR1 Polyclonal antibody (22150-1-AP)

    IF Staining of HeLa using 22150-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 22150-1-AP (PYCR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells
Positive IP detected inHeLa cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22150-1-AP is PYCR1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR1 fusion protein Ag17745
Full Name pyrroline-5-carboxylate reductase 1
Calculated Molecular Weight 319 aa, 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC022244
Gene Symbol PYCR1
Gene ID (NCBI) 5831
RRIDAB_11182280
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCR1,also named as P5CR1, belongs to the pyrroline-5-carboxylate reductase family. It is a housekeeping enzyme that catalyzes the last step in proline biosynthesis. PYCR1 can utilize both NAD and NADP, but has higher affinity for NAD. It is involved in the cellular response to oxidative stress. Mutation in PYCR1 will cause ARCL type II(ARCL2B ). Some mutation will cause DeBarsy syndrome (DBS) which is characterized by progeroid features, ophthalmological abnormalities, intrauterine growth retardation, and cutis laxa. The MW of PYCR1 is about 33-35kd. This antibody may have cross reaction to PYCR2 due to the high homology.

Protocols

Product Specific Protocols
WB protocol for PYCR1 antibody 22150-1-APDownload protocol
IF protocol for PYCR1 antibody 22150-1-APDownload protocol
IP protocol for PYCR1 antibody 22150-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Metab

Cancer-associated fibroblasts require proline synthesis by PYCR1 for the deposition of pro-tumorigenic extracellular matrix.

Authors - Emily J Kay
  • KD Validated
View Article