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  • Featured Product
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PYGB Polyclonal antibody

PYGB Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55380-1-AP

Synonyms

phosphorylase, glycogen; brain, PYGB

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of A549 using 55380-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of MCF-7 using 55380-1-AP

    WB result of PYGB antibody (55380-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PYGB transfected MCF-7 cells.

  • Western Blot (WB) analysis of HeLa cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of HeLa using 55380-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of MCF-7 using 55380-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of A549 using 55380-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of A549 using 55380-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-251 cells using PYGB Polyclonal antibody (55380-1-AP)

    WB analysis of U-251 using 55380-1-AP

    U-251 cells were subjected to SDS PAGE followed by western blot with 55380-1-AP (PYGB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PYGB Polyclonal antibody (55380-1-AP)

    IHC staining of mouse heart using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using PYGB Polyclonal antibody (55380-1-AP)

    IHC staining of mouse heart using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PYGB Polyclonal antibody (55380-1-AP)

    IHC staining of mouse brain using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PYGB Polyclonal antibody (55380-1-AP)

    IHC staining of mouse brain using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55380-1-AP (PYGB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human heart tissue using PYGB Polyclonal antibody (55380-1-AP)

    IHC staining of human heart using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using PYGB Polyclonal antibody (55380-1-AP)

    IHC staining of human heart using 55380-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 55380-1-AP (PYGB antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using PYGB Polyclonal antibody (55380-1-AP)

    IF Staining of A549 using 55380-1-AP

    Immunofluorescent analysis of A549 cells using 55380-1-AP (PYGB antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, HeLa cells, MCF-7 cells, U-251 cells
Positive IHC detected inmouse heart tissue, mouse brain tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

55380-1-AP targets PYGB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name phosphorylase, glycogen; brain
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 97-105 kDa
GenBank Accession NumberNM_002862
Gene Symbol PYGB
Gene ID (NCBI) 5834
RRIDAB_11182159
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYGB belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. This antibody is specific to PYGB. It has no cross reaction to PYGM and PYGL.

Protocols

Product Specific Protocols
WB protocol for PYGB antibody 55380-1-APDownload protocol
IHC protocol for PYGB antibody 55380-1-APDownload protocol
IF protocol for PYGB antibody 55380-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Front Mol Neurosci

Brain-Type Glycogen Phosphorylase Is Crucial for Astrocytic Glycogen Accumulation in Chronic Social Defeat Stress-Induced Depression in Mice.

Authors - Yuanyuan Zhu
View Article
human

J Immunol

Polarization and β-Glucan Reprogram Immunomodulatory Metabolism in Human Macrophages and Ex Vivo in Human Lung Cancer Tissues

Authors - Teresa W-M Fan
View Article