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KD/KO Validated

RAB14 Polyclonal antibody

Name: RAB14 Polyclonal antibody
Brand: Proteintech
SKU: 15662-1-AP

RAB14 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15662-1-AP

Synonyms

FBP, RAB 14, RAB14, Ras related protein Rab 14

Validation Data Gallery

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AllWBIHC

WB analysis of mouse brain using 15662-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 15662-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 15662-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 15662-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 15662-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 15662-1-AP (RAB14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human ovary tumor using 15662-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human ovary tumor using 15662-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15662-1-AP (RAB14 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 15662-1-AP

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Tested Applications

Positive WB detected in	mouse brain tissue, mouse kidney tissue, HepG2 cells, Raji cells, human brain tissue
Positive IHC detected in	human ovary tumor tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 10 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

15662-1-AP targets RAB14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RAB14 fusion protein Ag8092
Full Name	RAB14, member RAS oncogene family
Calculated Molecular Weight	215 aa, 24 kDa
Observed Molecular Weight	24 kDa
GenBank Accession Number	BC006081
Gene Symbol	RAB14
Gene ID (NCBI)	51552
RRID	AB_2173630
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB14 antibody 15662-1-AP	Download protocol
IHC protocol for RAB14 antibody 15662-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IHC	Cell Physiol Biochem Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer. Authors - Bo Guo View Article
human	IHC,WB	Onco Targets Ther Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers. Authors - Jun Zhang View Article
human	WB	PLoS One Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway. Authors - Bo Guo View Article
human	WB,IHC	Virchows Arch PMID: 30267303 Authors - Jinnian Ge KD Validated View Article
human	WB,IHC	Tumour Biol Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway. Authors - Rui Hou KD Validated View Article
human	WB	Eur Rev Med Pharmacol Sci STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14. Authors - C Du View Article

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Research Area

RAB14 Polyclonal antibody

RAB14 Polyclonal Antibody for IHC, WB,ELISA

Validation Data Gallery

WB analysis of mouse brain using 15662-1-AP

WB analysis of mouse kidney using 15662-1-AP

WB analysis of HepG2 using 15662-1-AP

WB analysis of Raji using 15662-1-AP

WB analysis of human brain using 15662-1-AP

IHC staining of human ovary tumor using 15662-1-AP

IHC staining of human ovary tumor using 15662-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Hypermethylation of miR-338-3p and Impact of its Suppression on Cell Metastasis Through N-Cadherin Accumulation at the Cell -Cell Junction and Degradation of MMP in Gastric Cancer.

Rab14 Overexpression Promotes Proliferation and Invasion Through YAP Signaling in Non-Small Cell Lung Cancers.

Rab14 Act as Oncogene and Induce Proliferation of Gastric Cancer Cells via AKT Signaling Pathway.

PMID: 30267303

Rab14 is overexpressed in ovarian cancers and promotes ovarian cancer proliferation through Wnt pathway.

STAT3-induced upregulation of lncRNA DUXAP8 functions as ceRNA for miR-577 to promote the migration and invasion in colorectal cancer through the regulation of RAB14.