RAB25 Polyclonal antibody

RAB25 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13189-1-AP

Synonyms

CATX 8, CATX8, RAB25, Ras related protein Rab 25



Tested Applications

Positive WB detected inHT-29 cells, Caco-2 cells, T-47D cells
Positive IHC detected inhuman renal cell carcinoma tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

13189-1-AP targets RAB25 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB25 fusion protein Ag3864
Full Name RAB25, member RAS oncogene family
Calculated Molecular Weight 213 aa, 23 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC033322
Gene Symbol RAB25
Gene ID (NCBI) 57111
RRIDAB_2877921
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAB25 antibody 13189-1-APDownload protocol
IHC protocol for RAB25 antibody 13189-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Transl Cancer Res

Rab25 acts as an oncogene and participates in the regulation of aerobic glycolysis via PKM2 in gastric adenocarcinoma

Authors - Tianyu Zheng

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elena (Verified Customer) (05-13-2022)

The antibody detected a single band at the expected molecular weight, under both reducing and non-reducing conditions

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:600
  • Cell Tissue Type: Ovarian cancer cells