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RAB31 Polyclonal antibody

RAB31 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15485-1-AP

Synonyms

Rab22B, RAB31, Ras related protein Rab 22B, Ras related protein Rab 31

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAB31 Polyclonal antibody (15485-1-AP)

    WB analysis using 15485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RAB31 Polyclonal antibody (15485-1-AP)

    WB analysis using 15485-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using RAB31 Polyclonal antibody (15485-1-AP)

    WB analysis of human placenta using 15485-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using RAB31 Polyclonal antibody (15485-1-AP)

    WB analysis of human placenta using 15485-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 15485-1-AP (RAB31 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB31 Polyclonal antibody (15485-1-AP)

    IHC staining of human liver cancer using 15485-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RAB31 Polyclonal antibody (15485-1-AP)

    IHC staining of human liver cancer using 15485-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15485-1-AP (RAB31 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, K-562 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15485-1-AP targets RAB31 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAB31 fusion protein Ag7833
Full Name RAB31, member RAS oncogene family
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC001148
Gene Symbol RAB31
Gene ID (NCBI) 11031
RRIDAB_2176911
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RAB31, also named as RAB22b, belongs to small GTP-binding proteins of the RAB family. Rab31 localizes in the trans-Golgi network (TGN) and endosomes. It is required for transport of mannose 6-phosphate receptors (MPRs) from the trans-Golgi network (TGN) to endosomes.

Protocols

Product Specific Protocols
WB protocol for RAB31 antibody 15485-1-APDownload protocol
IHC protocol for RAB31 antibody 15485-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Transl Med

From Bowen disease to cutaneous squamous cell carcinoma: eight markers were verified from transcriptomic and proteomic analyses

Authors - Tang Biao
View Article