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RABAC1 Polyclonal antibody

RABAC1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10542-1-AP

Synonyms

PRA1, PRA1 family protein 1, PRAF1, Rab acceptor 1 (prenylated), RABAC1, YIP3

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of HEK-293 cells using RABAC1 Polyclonal antibody (10542-1-AP)

    WB analysis of HEK-293 using 10542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RABAC1 Polyclonal antibody (10542-1-AP)

    WB analysis of HEK-293 using 10542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RABAC1 Polyclonal antibody (10542-1-AP)

    WB analysis of HEK-293 using 10542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RABAC1 Polyclonal antibody (10542-1-AP)

    WB analysis of HEK-293 using 10542-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human pancreas tissue using RABAC1 Polyclonal antibody (10542-1-AP)

    WB analysis of human pancreas using 10542-1-AP

    human pancreas tissue were subjected to SDS PAGE followed by western blot with 10542-1-AP (RABAC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RABAC1 Polyclonal antibody (10542-1-AP)

    IHC staining of human pancreas cancer using 10542-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RABAC1 Polyclonal antibody (10542-1-AP)

    IHC staining of human pancreas cancer using 10542-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10542-1-AP (RABAC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RABAC1 Polyclonal antibody (10542-1-AP)

    IF Staining of HEK-293 using 10542-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using RABAC1 Polyclonal antibody (10542-1-AP)

    IF Staining of HEK-293 using 10542-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 10542-1-AP (RABAC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, human pancreas tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10542-1-AP targets RABAC1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABAC1 fusion protein Ag0830
Full Name Rab acceptor 1 (prenylated)
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC008950
Gene Symbol RABAC1
Gene ID (NCBI) 10567
RRIDAB_2175639
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RABAC1 antibody 10542-1-APDownload protocol
IHC protocol for RABAC1 antibody 10542-1-APDownload protocol
IF protocol for RABAC1 antibody 10542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

BMC Neurosci

A role for prenylated rab acceptor 1 in vertebrate photoreceptor development.

Authors - Dickison Virginia M VM
View Article
mouse,humanWB,IF

PLoS One

Di-arginine and FFAT-like motifs retain a subpopulation of PRA1 at ER-mitochondria membrane contact sites.

Authors - Ameair Abu Irqeba
View Article
mouseWB

Biochim Biophys Acta Proteins Proteom

Phosphoproteomic response of cardiac endothelial cells to ischemia and ultrasound.

Authors - Uchenna Emechebe
View Article