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RABGAP1 Polyclonal antibody

RABGAP1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14223-1-AP

Synonyms

GAPCENA, RABGAP1, RP11 123N4.2, TBC1D11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RABGAP1 Polyclonal antibody (14223-1-AP)

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RABGAP1 Polyclonal antibody (14223-1-AP)

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RABGAP1 Polyclonal antibody (14223-1-AP)

    WB analysis using 14223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14223-1-AP (RABGAP1 antibody) at dilution of 1:14000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14223-1-AP targets RABGAP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RABGAP1 fusion protein Ag5462
Full Name RAB GTPase activating protein 1
Calculated Molecular Weight 122 kDa
Observed Molecular Weight 121 kDa
GenBank Accession NumberBC054492
Gene Symbol RABGAP1
Gene ID (NCBI) 23637
RRIDAB_2878030
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RABGAP1 antibody 14223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols