RAG2 Antibody 1 Publications

Rabbit Polyclonal| Catalog number: 11825-1-AP

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Freight/Packing

Con: 20 μg/150 μl

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Species specificity:
human, mouse

Positive WB detected in:
A375 cells, A375 cells, mouse thymus tissue

Positive IP detected in:
A375 cells

Positive IHC detected in:
human lymphoma tissue

Positive IF detected in:
Hela cells

Positive FC detected in:
HeLa cells

Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
recombination activating gene 2

Calculated molecular weight:
527aa,59 kDa

Observed molecular weight:
57-62 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
RAG2

Synonyms
RAG 2, RAG2
Background

Recombination activating gene 2(RAG2) is core part of the RAG complex(RAG1 and RAG2), which mediates the DNA cleavage phase during V(D)J recombination. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In the RAG complex, RAG2 is not the catalytic component but is required for all known catalytic activities mediated by RAG1. It probably acts as a sensor of chromatin state that recruits the RAG complex to H3K4me3


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