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RANGAP1 Polyclonal antibody

RANGAP1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11102-1-AP

Synonyms

Fug1, KIAA1835, RANGAP1, SD

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RANGAP1 Polyclonal antibody (11102-1-AP)

    WB analysis using 11102-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using RANGAP1 Polyclonal antibody (11102-1-AP)

    WB analysis of HEK-293 using 11102-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 11102-1-AP (RANGAP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using RANGAP1 Polyclonal antibody (11102-1-AP)

    IP experiment of HEK-293 using 11102-1-AP

    IP result of anti-RANGAP1 (IP:11102-1-AP, 4ug; Detection:11102-1-AP 1:300) with HEK-293 cells lysate 3600ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RANGAP1 Polyclonal antibody (11102-1-AP)

    IHC staining of human gliomas using 11102-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11102-1-AP (RANGAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using RANGAP1 Polyclonal antibody (11102-1-AP)

    IHC staining of human gliomas using 11102-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11102-1-AP (RANGAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RANGAP1 Polyclonal antibody (11102-1-AP)

    IF Staining of HepG2 using 11102-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 11102-1-AP (RANGAP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HepG2 cells using RANGAP1 Polyclonal antibody (11102-1-AP)

    FC experiment of HepG2 using 11102-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug RANGAP1 antibody (11102-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Tested Applications

Positive WB detected inSH-SY5Y cells, HEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11102-1-AP targets RANGAP1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RANGAP1 fusion protein Ag1563
Full Name Ran GTPase activating protein 1
Calculated Molecular Weight 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC014044
Gene Symbol RANGAP1
Gene ID (NCBI) 5905
RRIDAB_2176968
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RANGAP1 antibody 11102-1-APDownload protocol
IHC protocol for RANGAP1 antibody 11102-1-APDownload protocol
IF protocol for RANGAP1 antibody 11102-1-APDownload protocol
IP protocol for RANGAP1 antibody 11102-1-APDownload protocol
FC protocol for RANGAP1 antibody 11102-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF,CoIP

Cell Oncol (Dordr)

Hepatitis B virus core protein stabilizes RANGAP1 to upregulate KDM2A and facilitate hepatocarcinogenesis

Authors - Hong-Juan You
View Article
humanWB

J Proteomics

mTORC1 alters the expression of glycolytic genes by regulating KPNA2 abundances.

Authors - Xianwei Chen
View Article
human,mouseWB,IHC,IP

Dev Cell

Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma

Authors - Yan Gong
View Article