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RBAP48 Polyclonal antibody

RBAP48 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14557-1-AP

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RBAP48 Polyclonal antibody (14557-1-AP)

    WB analysis of HeLa using 14557-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using RBAP48 Polyclonal antibody (14557-1-AP)

    WB analysis of K-562 using 14557-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    WB analysis of mouse thymus using 14557-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14557-1-AP (RBAP48 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human ovary using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human ovary using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human kidney using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human kidney using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human placenta using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human placenta using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human testis using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human testis using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human brain using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human brain using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human spleen using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human spleen using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human lung using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using RBAP48 Polyclonal antibody (14557-1-AP)

    IHC staining of human lung using 14557-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RBAP48 Polyclonal antibody (14557-1-AP)

    IF Staining of HeLa using 14557-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14557-1-AP (RBAP48 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, mouse thymus tissue
Positive IHC detected inhuman ovary tissue, human brain tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14557-1-AP targets RBAP48 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBAP48 fusion protein Ag6110
Full Name retinoblastoma binding protein 4
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 40 kDa, 48-53 kDa
GenBank Accession NumberBC053904
Gene Symbol RBBP4
Gene ID (NCBI) 5928
RRIDAB_2177630
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBAP48 antibody 14557-1-APDownload protocol
IHC protocol for RBAP48 antibody 14557-1-APDownload protocol
IF protocol for RBAP48 antibody 14557-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols