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RBP5 Polyclonal antibody

RBP5 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 11843-1-AP

Synonyms

CRBP III, CRBP3, CRBPIII, HRBPiso, RBP5, Retinol binding protein 5

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using RBP5 Polyclonal antibody (11843-1-AP)

    WB analysis of human liver using 11843-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11843-1-AP (RBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of pig liver tissue using RBP5 Polyclonal antibody (11843-1-AP)

    WB analysis of pig liver using 11843-1-AP

    pig liver tissue were subjected to SDS PAGE followed by western blot with 11843-1-AP (RBP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat liver tissue using RBP5 Polyclonal antibody (11843-1-AP)

    WB analysis of rat liver using 11843-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 11843-1-AP (RBP5 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBP5 Polyclonal antibody (11843-1-AP)

    IHC staining of human liver cancer using 11843-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 11843-1-AP (RBP5 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RBP5 Polyclonal antibody (11843-1-AP)

    IHC staining of human liver cancer using 11843-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 11843-1-AP (RBP5 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RBP5 Polyclonal antibody (11843-1-AP)

    IF Staining of HepG2 using 11843-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11843-1-AP (RBP5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue, pig liver tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11843-1-AP targets RBP5 in WB, IHC, IF,ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBP5 fusion protein Ag2485
Full Name retinol binding protein 5, cellular
Calculated Molecular Weight 135 aa, 16 kDa
Observed Molecular Weight 16-18 kDa
GenBank Accession NumberBC029355
Gene Symbol RBP5
Gene ID (NCBI) 83758
RRIDAB_2085321
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RBP5 antibody 11843-1-APDownload protocol
IHC protocol for RBP5 antibody 11843-1-APDownload protocol
IF protocol for RBP5 antibody 11843-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols