• Featured Product
  • KD/KO Validated

REEP1 Polyclonal antibody

REEP1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17988-1-AP

Synonyms

C2orf23, receptor accessory protein 1, REEP1, SPG31



Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17988-1-AP targets REEP1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP1 fusion protein Ag12418
Full Name receptor accessory protein 1
Calculated Molecular Weight 201 aa, 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC064846
Gene Symbol REEP1
Gene ID (NCBI) 65055
RRIDAB_2178441
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for REEP1 antibody 17988-1-APDownload protocol
IHC protocol for REEP1 antibody 17988-1-APDownload protocol
IP protocol for REEP1 antibody 17988-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
IP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
humanWB

Autophagy

DIPK2A promotes STX17- and VAMP7-mediated autophagosome-lysosome fusion by binding to VAMP7B.

Authors - Xiaoyu Tian
mouseWB,IF

Hum Mol Genet

Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.

Authors - Benoît Renvoisé
  • KO Validated
humanWB

Neurobiol Dis

Disease-causing mutated ATLASTIN 3 is excluded from distal axons and reduces axonal autophagy.

Authors - Laura Behrendt
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu