REEP2 Polyclonal antibody

REEP2 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15684-1-AP

Synonyms

C5orf19, receptor accessory protein 2, REEP2, SGC32445



Tested Applications

Positive WB detected inmouse brain tissue, human heart tissue, mouse eye tissue, human skeletal muscle tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Positive FC detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15684-1-AP targets REEP2 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP2 fusion protein Ag8186
Full Name receptor accessory protein 2
Calculated Molecular Weight 252 aa, 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006218
Gene Symbol REEP2
Gene ID (NCBI) 51308
RRIDAB_2238326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for REEP2 antibody 15684-1-APDownload protocol
IHC protocol for REEP2 antibody 15684-1-APDownload protocol
IF protocol for REEP2 antibody 15684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
mouseWB

Cell Metab

Receptor-Mediated ER Export of Lipoproteins Controls Lipid Homeostasis in Mice and Humans.

Authors - Xiao Wang
human,mouseWB

Cell Res

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Authors - Pengli Zheng
mouseIP

Mol Psychiatry

Dysfunctional tubular endoplasmic reticulum constitutes a pathological feature of Alzheimer's disease.

Authors - M G Sharoar
mouseWB

Hum Mol Genet

Reep1 null mice reveal a converging role for hereditary spastic paraplegia proteins in lipid droplet regulation.

Authors - Benoît Renvoisé
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wanxia (Verified Customer) (07-16-2019)

Very good Reep2 antibody for WB and IF. It is so clean, almost no background.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: Mouse Brain