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REEP4 Polyclonal antibody

REEP4 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26650-1-AP

Synonyms

C8orf20, PP432, receptor accessory protein 4, REEP4

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using REEP4 Polyclonal antibody (26650-1-AP)

    WB analysis of mouse testis using 26650-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 26650-1-AP (REEP4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat testis tissue using REEP4 Polyclonal antibody (26650-1-AP)

    WB analysis of rat testis using 26650-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 26650-1-AP (REEP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using REEP4 Polyclonal antibody (26650-1-AP)

    IHC staining of human testis using 26650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26650-1-AP (REEP4 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using REEP4 Polyclonal antibody (26650-1-AP)

    IHC staining of human testis using 26650-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 26650-1-AP (REEP4 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using REEP4 Polyclonal antibody (26650-1-AP)

    IF Staining of HeLa using 26650-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 26650-1-AP (REEP4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26650-1-AP is REEP4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen REEP4 fusion protein Ag24569
Full Name receptor accessory protein 4
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC013048
Gene Symbol REEP4
Gene ID (NCBI) 80346
RRIDAB_2880589
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for REEP4 antibody 26650-1-APDownload protocol
IHC protocol for REEP4 antibody 26650-1-APDownload protocol
IF protocol for REEP4 antibody 26650-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

ER proteins decipher the tubulin code to regulate organelle distribution

Authors - Pengli Zheng
View Article
mouseWB

Hum Mol Genet

Transverse endoplasmic reticulum expansion in hereditary spastic paraplegia corticospinal axons.

Authors - Peng-Peng Zhu
View Article
humanIF

J Biol Chem

Comparative proximity biotinylation implicates the small GTPase RAB18 in sterol mobilization and biosynthesis

Authors - Robert S Kiss
View Article