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REPS2 Polyclonal antibody

REPS2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 20699-1-AP

Synonyms

Partner of RalBP1, POB1, RalBP1 interacting protein 2, REPS2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse brain tissue using REPS2 Polyclonal antibody (20699-1-AP)

    WB analysis of mouse brain using 20699-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using REPS2 Polyclonal antibody (20699-1-AP)

    WB analysis of human testis using 20699-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using REPS2 Polyclonal antibody (20699-1-AP)

    WB analysis of mouse kidney using 20699-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using REPS2 Polyclonal antibody (20699-1-AP)

    WB analysis of mouse brain using 20699-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of LNCaP cells using REPS2 Polyclonal antibody (20699-1-AP)

    WB analysis of LNCaP using 20699-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 20699-1-AP (REPS2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using REPS2 Polyclonal antibody (20699-1-AP)

    IF Staining of HepG2 using 20699-1-AP

    Immunofluorescent analysis of HepG2 cells, using REPS2 antibody 20699-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse brain tissue, human testis tissue, LNCaP cells, mouse kidney tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

20699-1-AP targets REPS2 in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name RALBP1 associated Eps domain containing 2
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa, 55 kDa, 30 kDa
GenBank Accession NumberNM_004726
Gene Symbol REPS2
Gene ID (NCBI) 9185
RRIDAB_10697669
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

REPS2, also named as POB1, is involved in growth factor signaling through its influence on the Ral signaling pathway. The antibody is specific to REPS2.

Protocols

Product Specific Protocols
WB protocol for REPS2 antibody 20699-1-APDownload protocol
IF protocol for REPS2 antibody 20699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Signal

REPS2 downregulation facilitates FGF-induced adhesion and migration in human lens epithelial cells through FAK/Cdc42 signaling and contributes to posterior capsule opacification.

Authors - Chan Wen
View Article