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RFFL Polyclonal antibody

RFFL Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 12687-1-AP

Synonyms

CARP 2, Caspase regulator CARP2, Fring, RFFL, RIFIFYLIN, RING finger protein 189, RING finger protein 34 like, RNF189, RNF34L



Tested Applications

Positive WB detected inmouse testis tissue, mouse thymus tissue
Positive IP detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12687-1-AP targets RFFL in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RFFL fusion protein Ag3380
Full Name ring finger and FYVE-like domain containing 1
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 37-41 kDa
GenBank Accession NumberBC028424
Gene Symbol RFFL
Gene ID (NCBI) 117584
RRIDAB_10644289
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RFFL antibody 12687-1-APDownload protocol
IP protocol for RFFL antibody 12687-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Competition for cysteine acylation by C16:0 and C18:0 derived lipids is a global phenomenon in the proteome

Authors - Hana Nůsková
humanWB,CoIP

Cell Death Dis

ETS-1-activated LINC01016 over-expression promotes tumor progression via suppression of RFFL-mediated DHX9 ubiquitination degradation in breast cancers

Authors - Ying Sun
  • KD Validated