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RIBC2 Polyclonal antibody

RIBC2 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15272-1-AP

Synonyms

C22orf11, RIBC2

Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    WB analysis of human brain using 15272-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    WB analysis of human brain using 15272-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15272-1-AP (RIBC2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    IHC staining of human colon cancer using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using RIBC2 Polyclonal antibody (15272-1-AP)

    IHC staining of human colon cancer using 15272-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 15272-1-AP (RIBC2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RIBC2 Polyclonal antibody (15272-1-AP)

    IF Staining of HeLa using 15272-1-AP

    Immunofluorescent analysis of Hela cells, using RIBC2 antibody 15272-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15272-1-AP targets RIBC2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RIBC2 fusion protein Ag7385
Full Name RIB43A domain with coiled-coils 2
Calculated Molecular Weight 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC003024
Gene Symbol RIBC2
Gene ID (NCBI) 26150
RRIDAB_2179714
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RIBC2 antibody 15272-1-APDownload protocol
IHC protocol for RIBC2 antibody 15272-1-APDownload protocol
IF protocol for RIBC2 antibody 15272-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols