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RIM2-Specific Polyclonal antibody

RIM2-Specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20093-1-AP

Synonyms

KIAA0751, OBOE, Rab 3 interacting molecule 2, Rab 3 interacting protein 3, RAB3IP3, RIM 2, RIM2, RIM2-Specific, RIMS2

Validation Data Gallery

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  • Western Blot (WB) analysis of L02 cells using RIM2-Specific Polyclonal antibody (20093-1-AP)

    WB analysis of L02 using 20093-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 20093-1-AP (RIM2-Specific antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RIM2-Specific Polyclonal antibody (20093-1-AP)

    IHC staining of mouse brain using 20093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using RIM2-Specific Polyclonal antibody (20093-1-AP)

    IHC staining of mouse brain using 20093-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20093-1-AP (RIM2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inL02 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20093-1-AP targets RIM2-Specific in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name regulating synaptic membrane exocytosis 2
Calculated Molecular Weight 160 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberNM_001100117
Gene Symbol RIMS2
Gene ID (NCBI) 9699
RRIDAB_10666429
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RIMS2, also named as KIAA0751, RAB3IP3 and RIM2, is a rab effector which involved in exocytosis. RIMS2 may act as scaffold protein. This antibody is specific to RIMS2. It recognizes all the isoforms except isoform7 of RIMS2.

Protocols

Product Specific Protocols
WB protocol for RIM2-Specific antibody 20093-1-APDownload protocol
IHC protocol for RIM2-Specific antibody 20093-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tongbin (Verified Customer) (08-25-2020)

This antibody works well in detecting Rims2 in mouse tissue samples.

  • Applications: Western Blot
  • Cell Tissue Type: Heart