- Featured Product
- KD/KO Validated
RIOK3 Polyclonal antibody
RIOK3 Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 13593-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, mouse testis tissue, MCF-7 cells, rat testis tissue, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
Product Information
13593-1-AP targets RIOK3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RIOK3 fusion protein Ag4497 |
| Full Name | RIO kinase 3 (yeast) |
| Calculated Molecular Weight | 516 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC039729 |
| Gene Symbol | RIOK3 |
| Gene ID (NCBI) | 8780 |
| RRID | AB_2178105 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RIOK3(Serine/threonine-protein kinase RIO3) is initially characterized as a homolog of Aspergillus nidulans sudD and showed down-regulation at the invasive front of malignant melanomas.On Western blots, human RIOK3 migrated as a 60-kD protein. Northern blot analysis indicated that the approximately 4-kb RIOK3 mRNA is expressed widely in human tissues, with additional smaller transcripts present in testis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| IHC protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| IF protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| IP protocol for RIOK3 antibody 13593-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection. | ||
Genes Dev miR-191 regulates mouse erythroblast enucleation by down-regulating Riok3 and Mxi1. | ||
Biomedicines RIOK2 Inhibitor NSC139021 Exerts Anti-Tumor Effects on Glioblastoma via Inducing Skp2-Mediated Cell Cycle Arrest and Apoptosis. | ||
Cell Rep Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.
|
