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  • Featured Product
  • KD/KO Validated

RNF7 Polyclonal antibody

RNF7 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

Cat no : 11905-1-AP

Synonyms

CKBBP1, CKII beta binding protein 1, Rbx2, Regulator of cullins 2, RING box protein 2, ring finger protein 7, RNF7, ROC2, SAG

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using RNF7 Polyclonal antibody (11905-1-AP)

    WB analysis of human liver using 11905-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using RNF7 Polyclonal antibody (11905-1-AP)

    WB analysis of human testis using 11905-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RNF7 Polyclonal antibody (11905-1-AP)

    WB analysis of human heart using 11905-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skeletal muscle tissue using RNF7 Polyclonal antibody (11905-1-AP)

    WB analysis of human skeletal muscle using 11905-1-AP

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using RNF7 Polyclonal antibody (11905-1-AP)

    WB analysis of human heart using 11905-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 11905-1-AP (RNF7 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse heart tissue using RNF7 Polyclonal antibody (11905-1-AP)

    IP experiment of mouse heart using 11905-1-AP

    IP result of anti-RNF7 (IP:11905-1-AP, 4ug; Detection:11905-1-AP 1:600) with mouse heart tissue lysate 4400ug.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF7 Polyclonal antibody (11905-1-AP)

    IF Staining of HepG2 using 11905-1-AP

    Immunofluorescent analysis of HepG2 cells, using RNF7 antibody 11905-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using RNF7 Polyclonal antibody (11905-1-AP)

    IF Staining of mouse heart using 11905-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using RNF7 antibody (11905-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue, human heart tissue, human skeletal muscle tissue, human testis tissue
Positive IP detected inmouse heart tissue
Positive IF detected inmouse heart tissue, HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11905-1-AP targets RNF7 in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF7 fusion protein Ag2553
Full Name ring finger protein 7
Calculated Molecular Weight 113 aa, 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC008627
Gene Symbol RNF7
Gene ID (NCBI) 9616
RRIDAB_10697836
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF (ring finger protein 7 ) is a novel zinc RING finger protein which is redox responsive and protects mammalian cells from apoptosis.It is shown to be localised in the cytoplasmand nucleus of the cell and is expressed in the heart, liver, skeletal muscle predominantly. RNF7 forms oligomers(55 kDa),dimer(28 kDa) and monomer(13 kDa) in the presence of different concentrations of the reductant.

Protocols

Product Specific Protocols
WB protocol for RNF7 antibody 11905-1-APDownload protocol
IF protocol for RNF7 antibody 11905-1-APDownload protocol
IP protocol for RNF7 antibody 11905-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell Proteomics

Neddylation-Cullin 2-RBX1 E3 ligase axis targets tumor suppressor RhoB for degradation in liver cancer.

Authors - Junfeng Xu
  • KD Validated
View Article
humanWB

FASEB J

PMID: 30844312

Authors - Tengyu Ko
  • KD Validated
View Article