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RNGTT Polyclonal antibody

RNGTT Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 12430-1-AP

Synonyms

CAP1A, GTase, GTP RNA guanylyltransferase, hCAP, HCAP1, HCE, HCE1, mRNA 5 triphosphatase, mRNA capping enzyme, RNGTT, TPase mRNA guanylyltransferase

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using RNGTT Polyclonal antibody (12430-1-AP)

    WB analysis of human kidney using 12430-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 12430-1-AP (RNGTT antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using RNGTT Polyclonal antibody (12430-1-AP)

    IP experiment of mouse kidney using 12430-1-AP

    IP result of anti-RNGTT (IP:12430-1-AP, 4ug; Detection:12430-1-AP 1:500) with mouse kidney tissue lysate 4000ug.

Tested Applications

Positive WB detected inhuman kidney tissue
Positive IP detected inmouse kidney tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12430-1-AP targets RNGTT in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNGTT fusion protein Ag3105
Full Name RNA guanylyltransferase and 5'-phosphatase
Calculated Molecular Weight 597 aa, 69 kDa
Observed Molecular Weight 69 kDa
GenBank Accession NumberBC019954
Gene Symbol RNGTT
Gene ID (NCBI) 8732
RRIDAB_2181385
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for RNGTT antibody 12430-1-APDownload protocol
IP protocol for RNGTT antibody 12430-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols