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  • Featured Product
  • KD/KO Validated

RNPC3 Polyclonal antibody

RNPC3 Polyclonal Antibody for IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 25820-1-AP

Synonyms

KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein

Validation Data Gallery

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  • Western Blot (WB) analysis of 3T3-L1 cells using RNPC3 Polyclonal antibody (25820-1-AP)

    WB analysis of 3T3-L1 using 25820-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25820-1-AP (RNPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse spleen tissue using RNPC3 Polyclonal antibody (25820-1-AP)

    WB analysis of mouse spleen using 25820-1-AP

    mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25820-1-AP (RNPC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse spleen tissue using RNPC3 Polyclonal antibody (25820-1-AP)

    IP experiment of mouse spleen using 25820-1-AP

    IP result of anti-RNPC3 (IP:25820-1-AP, 4ug; Detection:25820-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

Tested Applications

Positive WB detected in3T3-L1 cells, mouse spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25820-1-AP targets RNPC3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNPC3 fusion protein Ag22957
Full Name RNA-binding region (RNP1, RRM) containing 3
Calculated Molecular Weight 517 aa, 59 kDa
Observed Molecular Weight 62-65 kDa
GenBank Accession NumberBC010697
Gene Symbol RNPC3
Gene ID (NCBI) 55599
RRIDAB_2880254
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNPC3 antibody 25820-1-APDownload protocol
IP protocol for RNPC3 antibody 25820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS Genet

Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA.

Authors - Jens Verbeeren
View Article
mouseWB

RNA

Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice.

Authors - Karen Doggett
  • KO Validated
View Article