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RNPEP Polyclonal antibody

RNPEP Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14764-1-AP

Synonyms

Aminopeptidase B, AP B, APB, Arginine aminopeptidase, Arginyl aminopeptidase, RNPEP

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using RNPEP Polyclonal antibody (14764-1-AP)

    WB analysis of HeLa using 14764-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RNPEP Polyclonal antibody (14764-1-AP)

    WB analysis of HepG2 using 14764-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RNPEP Polyclonal antibody (14764-1-AP)

    WB analysis of A549 using 14764-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A549 cells using RNPEP Polyclonal antibody (14764-1-AP)

    IP experiment of A549 using 14764-1-AP

    IP result of anti-RNPEP (IP:14764-1-AP, 4ug; Detection:14764-1-AP 1:500) with A549 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNPEP Polyclonal antibody (14764-1-AP)

    IHC staining of human lung cancer using 14764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using RNPEP Polyclonal antibody (14764-1-AP)

    IHC staining of human lung cancer using 14764-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHL-60 cells, HepG2 cells, A549 cells, HeLa cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected inA549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

14764-1-AP targets RNPEP in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNPEP fusion protein Ag6526
Full Name arginyl aminopeptidase (aminopeptidase B)
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC001064
Gene Symbol RNPEP
Gene ID (NCBI) 6051
RRIDAB_2254005
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RNPEP antibody 14764-1-APDownload protocol
IHC protocol for RNPEP antibody 14764-1-APDownload protocol
IP protocol for RNPEP antibody 14764-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Autism

RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.

Authors - Natasha L Pacheco
View Article