RPE Polyclonal antibody

RPE Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 12168-2-AP

Synonyms

ribulose 5 phosphate 3 epimerase, Ribulose phosphate 3 epimerase, RPE, RPE2 1



Tested Applications

Positive WB detected inHEK-293T cells, mouse thymus tissue, HeLa cells, K-562 cells
Positive IF detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12168-2-AP targets RPE in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPE fusion protein Ag2810
Full Name ribulose-5-phosphate-3-epimerase
Calculated Molecular Weight 228 aa, 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016764
Gene Symbol RPE
Gene ID (NCBI) 6120
RRIDAB_2253939
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPE antibody 12168-2-APDownload protocol
IF protocol for RPE antibody 12168-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Rep

mTOR Overcomes Multiple Metabolic Restrictions to Enable HIV-1 Reverse Transcription and Intracellular Transport.

Authors - Harry E Taylor
humanWB

Nat Metab

Salvage of ribose from uridine or RNA supports glycolysis in nutrient-limited conditions

Authors - Owen S Skinner
  • KO Validated