RPIA Polyclonal antibody
RPIA Polyclonal Antibody for IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, ELISA
Conjugate
Unconjugated
Cat no : 13010-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse spleen tissue |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
13010-1-AP targets RPIA in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | RPIA fusion protein Ag3699 |
Full Name | ribose 5-phosphate isomerase A |
Calculated Molecular Weight | 311 aa, 33 kDa |
Observed Molecular Weight | 33 kDa |
GenBank Accession Number | BC015529 |
Gene Symbol | RPIA |
Gene ID (NCBI) | 22934 |
RRID | AB_10638301 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPIA(Ribose-5-phosphate isomerase) is also named as RPI and belongs to the ribose 5-phosphate isomerase family. It is involved in pentose-phosphate shunt, non-oxidative branch. It is an enzyme in the pentose-phosphate pathway, appears to be determined by a single structural locus and is probably a dimer(PMID:7396409).
Protocols
Product Specific Protocols | |
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WB protocol for RPIA antibody 13010-1-AP | Download protocol |
IF protocol for RPIA antibody 13010-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |